Initial dose based on previous asthma drug therapy and disease severity; 100 mcg via oral inhalation once daily is the usual recommended starting dose for patients not on an inhaled corticosteroid. After 2 weeks of therapy, if asthma symptoms are uncontrolled, increase dose to 200 mcg via oral inhalation once daily. Max: 200 mcg once daily. Administer at the same time each day. The maximum beneficial effect may not be achieved for up to 2 weeks or longer after starting treatment. Titrate to the lowest effective dose once asthma stability is achieved.
The cooling zone temperature in these cases can be established beforehand with the aid of a melting point measuring instrument equipped with a opticaly microscope (. Mettler's melting/boiling point meter Model FP-80 or FP-82HT equipped with a polarizing microscope), a differential scanning calorimeter (DSC) or the like. Thus, in the case of a melting point measuring instrument equipped with a opticaly microscope, the method can be used which comprises melting the medicinal substance on a slide glass, cooling it to find the temperature at which metastable crystals are formed and using the particular temperature as the cooling zone temperature.
The neurotoxin MPTP had been known earlier to cause PD-like symptoms (in humans and other primates, though not in rats) by interfering with complex I in the electron transport chain and killing dopaminergic neurons in the substantia nigra. However, further studies involving MPTP have failed to show development of Lewy bodies , a key component to PD pathology. Therefore, the mechanism behind MPTP as it relates to Parkinson's disease is not fully understood.  Because of these developments, rotenone was investigated as a possible Parkinson-causing agent. Both MPTP and rotenone are lipophilic and can cross the blood–brain barrier .